A25743 ™ SYBR® Green Master Mix

规格  

Detection Method:	SYBR
Platform:	7500 Fast Dx System, Real-Time PCR Systems or Thermal Cyclers, 7300 System, 7500 Fast System, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Bio-Rad CFX Connect™, 7900HT Fast System, 7700 System, StepOne™ System, StepOnePlus™ System
Reaction Speed:	Fast or Standard
PCR Method:	qPCR
Sample Type (General):	DNA
Polymerase:	Dual-Lock Taq DNA Polymerase
Product Size:	50 mL
Shipping Condition:	Wet Ice
Number of Reactions:	5000 reactions

  内容及储存     描述   PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range