产品简介
™ SYBR® Green Master Mix7500 Fast Dx System, Real-Time PCR Systems or Thermal Cyclers, 7300 System, 7500 Fast System, ViiA™ 7 System, Bio-Rad CFX384
公司简介
北京杰辉博高生物技术有限公司依托国家重点实验室建立,是由具有资深生命科学、生物技术背景及留学归国人员共同创办的高新技术企业。
公司专业经营生物科学领域的实验仪器、研究试剂和实验室耗材,致力于向国内外生物科学研究人员提供一流的服务。
目前,公司推出的产品涵盖了生物化学、分子生物学、免疫学、细胞生物学等相关领域,因优质稳定、性价比高等特点在广大科研机构、研究院校及相关企业中赢得了良好的声誉,从而建立了立体的营销网络和拥有广泛的客户资源。
公司向广大用户提供优质产品的同时,始终将客户服务和技术支持放在比产品销售更重要的位置上,公司聘请了专职、兼职技术支持人员,为客户提供专业性、个性化的技术服务。
公司秉承"先做人,后做事"的理念,坚持"以质量为生命,以客户为中心"的宗旨,把最优质的产品和最贴心的服务奉献给广大客户。
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产品说明
Detection Method:
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SYBR
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Platform:
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7500 Fast Dx System, Real-Time PCR Systems or Thermal Cyclers, 7300 System, 7500 Fast System, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Bio-Rad CFX Connect™, 7900HT Fast System, 7700 System, StepOne™ System, StepOnePlus™ System
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Reaction Speed:
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Fast or Standard
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PCR Method:
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qPCR
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Sample Type (General):
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DNA
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Polymerase:
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Dual-Lock Taq DNA Polymerase
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Product Size:
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1 mL
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Shipping Condition:
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Wet Ice
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Number of Reactions:
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100 reactions
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内容及储存
The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100 20-µL reactions (10 µL Master Mix per reaction).
Store at 2–8°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
描述
PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.
PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves of the time, consistent with highly specific amplification.
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