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价格:电议
所在地:上海
型号:
更新时间:2012-08-24
浏览次数:1265
公司地址:上海市徐汇区石龙路345弄7号楼401室
唐小姐(女士)
异硫氰酸荧光素 (fluorescein isothiocyanate, FITC) FITC纯品为黄色或橙黄色结晶粉末,易溶于水和酒精溶剂。有两种异构体,其中异构体Ⅰ型在效率、稳定性与蛋白质结合力等方面都更优良。FITC分子量为389.4,zui大吸收光波长为490~495nm,zui大发射光波长为520~530nm,呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年,是目前应用广泛的荧光素。其主要优点是人眼对黄绿色较为敏感,通常切片标本中的绿色荧光少于红色。
Description
Application Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.
Properties
Sigma F7250 Fluorescein isothiocyanate isomer I
suitable for protein labeling, ≥90% (HPLC), powder
Synonym: FITC, Fluorescein 5-isothiocyanate
Molecular Weight: 389.38
Beilstein Registry Number: 1407295
CAS Number: 3326-32-7
Linear Formula: C21H11NO5S
EC Number: 222-042-0
MDL number: MFCD00005063
PubChem Substance ID: 24894933
assay: ≥90% (HPLC)
form powder
fluorescence λex 492 nm; λem 518 nm (green)
mp >360 °C(lit.)
suitability suitable for protein labeling
storage temp. 2-8°C
Safety
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 42/43
Safety Statements 22-36/37-45
WGK Germany 3