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- 产品/服务:MG 人脑星形胶质母细胞瘤
- 型 号:U-118
- 品 牌:上海复祥生物
- 单 价:面议
- 更新日期:2024-03-23
- 有效期至:长期有效
- 浏览次数:1319
- 立即询价
U-118 MG 人脑星形胶质母细胞瘤ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件...
U-118 MG (ATCC® HTB-15™)
Organism Homo sapiens, human
Tissue brain
Cell Type Glioblastoma
Product Format frozen
Morphology mixed
Culture Properties adherent
Biosafety Level 1
Disease classified as grade IV as of 2007, glioblastoma; astrocytoma
Age 50 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
tion U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-16) by J. Po
Clinical Data 50 years
Caucasian
male
Antigen ex
Tumorigenic Yes
Effects Yes, in nude mice inoculated subcutaneously with 10(7) cells
(Tumors developed within 21 days at frequency (5/5).)
Comments NOTE: The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
Mycoplasma co
Complete Growth Medium The ba
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additio
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapot temperature
Culture Conditions Temperature: 37°C
STR Profile Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
Isoenzymes AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 2
Name of Depositor J Po
Year of Origin 1966
References Beckman G, et al. G-6-PD and PGM phenotypes of 16 co
Fogh J, et al. Absence of HeLa cell co
Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Po
Pollack MS, et al. HLA-A, B, C and DR alloantigen ex
Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013
Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
Tumors developed within 21 days at frequency (5/5).
References Beckman G, et al. G-6-PD and PGM phenotypes of 16 co
Fogh J, et al. Absence of HeLa cell co
Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Po
Pollack MS, et al. HLA-A, B, C and DR alloantigen ex
Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013
Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
Tumors developed within 21 days at frequency (5/5).
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