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- 产品/服务:人膀胱移行细胞**瘤细胞
- 型 号:HTB-2™
- 品 牌:上海复祥生物
- 单 价:面议
- 更新日期:2024-03-23
- 有效期至:长期有效
- 浏览次数:1140
- 立即询价
RT4 人膀胱移行细胞**瘤细胞 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件...
RT4 (ATCC® HTB-2™)
Organism Homo sapiens, human
Tissue urinary bladder
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease transitio
Age 63 years
Gender male
Ethnicity Caucasian
Applications This cell line is a transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy me
Clinical Data 63 years
Caucasian
male
Antigen ex
Tumorigenic Yes
Effects Yes, in cheek pouch of steroid-treated hamsters
Comments A co
Complete Growth Medium The ba
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additio
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
STR Profile Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9,12
THO1: 9,9.3
TPOX: 8,11
vWA: 14,17
Isoenzymes AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1-2
PGM3, 1-2
Name of Depositor C Rigby, LM Franks
References O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen ex
O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436
Fogh J, et al. Absence of HeLa cell co
Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
Rigby CC, Franks LM. A human tissue culture cell line from a transitio
Pollack MS, et al. HLA-A, B, C and DR alloantigen ex
References O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen ex
O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436
Fogh J, et al. Absence of HeLa cell co
Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
Rigby CC, Franks LM. A human tissue culture cell line from a transitio
Pollack MS, et al. HLA-A, B, C and DR alloantigen ex
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