周期素D相互作用蛋白1抗体说明书

产品名称  周期素D相互作用蛋白1抗体
英文名称  Anti-DMTF1     
浓    度  1mg/1ml     
规 格  0.2ml/200μg
抗体来源  Rabbit      
克隆类型  polyclonal     
交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit       
产品类型  一抗        
研究领域  细胞生物 细胞周期蛋白 转录调节因子 表观遗传学      
蛋白分子量  predicted molecular weight: 84kDa     
性    状  Lyophilized or Liquid     
免 疫 原  KLH conjugated synthetic peptide derived from human DMTF1     
亚    型  IgG     
纯化方法  affinity purified by Protein A     
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4     
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500    
（石蜡切片需做抗原修复）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest. Binds to the consensus sequence 5'-CCCG[GT]ATGT-3'. Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.

Function : Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity). Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity). Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.

Subunit : Interacts with the D-type cyclins CCND1, CCND2 and CCND3 (By similarity). Interaction with D-type cyclins may modulate transcriptional activation by this protein (By similarity).

Subcellular Location : Nucleus.
Tissue Specificity : Expressed at relatively low levels in colonic mucosa, ovary, peripheral leukocytes, prostate and small intestine, and at higher levels in spleen, testis and thymus. Expressed in multiple regions of the brain and CNS including amygdala, caudate, corpus callosum, hippocampus, substantia nigra and subthalamic nucleus. Isoform 1 is the predominant isoform in monocytes, macrophages and neutrophils, isoform 2 is most strongly expressed in peripheral blood leukocytes and quiescent CD34 positive cells, and isoform 3 is expressed at low levels in all hematopoietic cell types. expression is frequently reduced in non-small-cell lung carcinomas (NSCLC) due to hemizygous gene deletion, strongly suggesting that this locus is haploinsufficient for tumor suppression. Loss of this locus frequently occurs in tumors which retain wild-type CDKN2A/ARF and p53/TP53 loci. Hemizygous gene deletion has also been observed in leukemic blasts from patients with abnormalities of the long arm of chromosome 7.

Post-translational modifications : Phosphorylated by the cyclin-D2/CDK4, cyclin-D3/CDK4 and cyclin-D2/CDK6 complexes and to a lesser extent by the cyclin-D1/CDK4 complex (By similarity).
Similarity : Belongs to the DMTF1 family.
Contains 1 HTH myb-type DNA-binding domain.
Contains 2 Myb-like domains.
Database links : UniProtKB/Swiss-Prot: Q9Y222.1