BMEG02 hp-Vector表达系统megaterium芽孢杆菌

hp-Vector表达系统megaterium芽孢杆菌hp-Vector Expression Systems for Bacillus megateriumFeatures: High performance vector

 北京毕特博生物技术有限责任公司（Beijing Bitab Biotechnology Co.,Ltd）是一家成立于1994年8月2日的经营生命科学用品的高科技留学生企业。她以代理国际著名公司生产的生物试剂、生物仪器、设备和耗材等产品为主, 并兼营其它相关业务：提供转基因服务，协助科研设计和数据处理，以及组织国内和国际学术会议，提供相关网络服务等。 目前，毕特博生物代理和经销的外国公司主要供货商有Lonza(原Cambrex)、MP Biomedicals（MP、Qbiogene、ICN）、Alomone、Alexis、Prospec、Fitzgerald、Cryologic、Mobitec、Biosight（肽标记试剂盒）、Eurobiosciences(CD抗体）等,涉及的产品范围主要有：细胞生物学、分子生物学、免疫学、食品与水质检测、实验室设备等。 在此我们再次感谢广大用户曾经或即将给予我们工作的理解和支持，并竭诚欢迎各地有志之士加盟毕特博生物或分销毕特博生物所代理的产品！

hp-Vector表达系统megaterium芽孢杆菌

hp-Vector Expression Systems for Bacillus megaterium

Features:

High performance vectors with optimized sequence

Protein yield up to 10 times enhanced compared to protein expression with basic plasmid

Plasmids with established MCS

Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)

Secretion with LipA or YocH signal peptide up to 9 fold increased

optimized high performance (hp) vectors for Bacillus megaterium!

MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence and a 6xHis-tag.

With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated and efficiently inducible xylose operon.

Versatile system with a wide range of vectors

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

B. megaterium protoplasts specifically optimized for transformation

The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Order Information, shipping and storage

Please click on the respective order # for further details.

order# description amoun（订购货号信息）

BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1)

Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl

BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl

BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg

BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg

BMEG30 p3S623hp, shuttle vector, lyophilized DNA 10 μg

BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg

BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg

shipped at RT, protoplasts shipped on dry ice

store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C

All vectors are E. coli / B. megaterium shuttle vectors

Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.

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