CRL-5826 NCI-H226 人肺癌细胞株

CRL-5826 NCI-H226 人肺癌细胞株 ，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和优培养条件

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 CRL-5826 NCI-H226 人肺癌细胞株 
ATCC® Number: CRL-5826™ 
Designations: NCI-H226 [H226] 
Depositors: AF Gazdar, JD Minna 
Biosafety Level: 1 
Shipped: frozen 
Medium & Serum: See Propagation 
Growth Properties: adherent 
Organism: Homo sapiens (human) 
Morphology: Epithelial


Source: Organ: lung 
Disease: squamous cell carcinoma; mesothelioma [23570] 
Derived from metastatic site: pleural effusion 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. 
Isolation: Isolation date: March, 1980 
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 10,11 
D13S317: 13,14 
D16S539: 9,12 
D5S818: 11,12 
D7S820: 8,10 
THO1: 8,9.3 
TPOX: 8,11 
vWA: 17 
Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25) 
Gender: male 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Subculturing: Protocol: 
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37C.

Subcultivation Ratio: 1:4 to 1:8 is recommended 
Medium Renewal: Every 2 to3 days 
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. 

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