MX4453 （MDC） 单丹磺酰戊二胺

产品名称

产品编号

CAS NO.

规格

价格（元）

Dansylcadaverine (MDC) 单丹磺酰戊二胺

MX4453-10MG

10121-91-2

10mg

128

Dansylcadaverine (MDC) 单丹磺酰戊二胺

MX4453-50MG

10121-91-2

50mg

388

文献1：Contento AL et al. Visualization of autophagy in Arabidopsis using the fluorescent dye monodansylcadaverine and a GFP-AtATG8e fusion protein. Plant J. 2005 May;42(4):598-608.

Methods (MDC staining): Arabidopsis cells were stained with a 0.05 mM final concentration of MDC in PBS for 10 min. When staining protoplasts, PBS was supplemented with 0.4 M mannitol. Cells were washed with PBS two times to remove excess MDC. Fluorescence of suspension cells was measured using a Hitachi F-2000 fluorescence spectrophotometer with anexcitation wavelength of 335 nm and an emission wavelength of 508 nm. The intensity of MDC staining was expressed in relative fluorescence per gram of fresh weight, as a percentage of the 0-h control.

Results: MDC stains autophagosomes in Arabidopsis, as it does in mammaliancells.

文献2：Ma KG et al. Autophagy is activated in compression-induced cell degeneration and is mediated by reactive oxygen species in nucleus pulposus cells exposed to compression. Osteoarthritis Cartilage. 2013 Dec;21(12):2030-8. PMID: 24120490

Methods (MDC staining): Cells were seeded in 24-well culture plates and treated with compression for 0, 12, 24, 36 and 48 h. The autophagy inhibitor 3-MA was applied to assay the effect of compression on IVD cell autophagy. At each time point following treatment, cells were rinsed in PBS (three times for 5 min), incubated with a 0.05 mM solution of MDC dye (diluted from the 0.5 M stock solution in DMSO) at 37C for 15 min, and then washed three times in PBS.

货号

名称

规格

目录价（元）

MX4454-25G

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25g

88

MX4455-1MG

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1mg

528

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Sulforhodamine B 磺酰罗丹明B

5g

1748

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Sulforhodamine B Sodium Salt 磺酰罗丹明B钠盐

5g

98

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Sulforhodamine 101 磺酰罗丹明101

25mg

450

MX4462-10MG

Sulforhodamine 101 acid chloride (Texas Red) 磺酰罗丹明101磺酰氯

10mg

1650

MX3208-5MG

Rhodamine 123 罗丹明123（线粒体膜电位荧光探针）

5mg

280

MX4012-10MG

Rhodamine B Octadecyl Ester Perchlorate 罗丹明B十八烷酯高氯酸盐

10mg

1599

MX4013-10MG

Octadecyl Rhodamine B Chloride (R18) 十八烷基罗丹明B氯盐

10mg

3328