内容及储存
The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 X 50 mL tube, sufficient for 5000 20-µL reactions (10 µL Master Mix per reaction).
Store at 2–8°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
描述
PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene ex
pression analysis.
PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments
Exceptio
nal specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through co
ntrol of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help co
ntrol its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment e
valuating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves of the time, co
nsistent with highly specific amplification.
Tight reproducibility in CTs over a wide dynamic range