MZ2504 Calyculin A 花萼海绵体诱癌素A

产品名称

产品编号

CAS NO.

规格

价格（元）

Calyculin A 花萼海绵体诱癌素A

MZ2504-25UG

101932-71-2

25 µg

1446

Calyculin A 花萼海绵体诱癌素A

MZ2504-50UG

101932-71-2

50 µg

2486

Calyculin A 花萼海绵体诱癌素A

MZ2504-100UG

101932-71-2

100 µg

3946

文献1，Hudák R et al. The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Plaet Activation, and Thrombin Generation. Biomed Res Int. 2017:9795271. PMID: 28680886

体外研究：

细胞类型（Cell type）：Plaet-rich plasma (PRP) sample

药物配制（Preparation）：Calyculin-A (CLA) was dissolved in DMSO

实验方法（Assay）：PRP (110μL) was preincubated with either HEPES buffer containing 0.5% DMSO as control or the protein phosphatase inhibitor CLA (50nM), for 30 minutes at 37°C in a water bath. After preincubation, plaets were activated either by TRAP (20μM) or by thiomersal (200mM) for 15 minutes at 37°C in a water bath. Then PRP (5μL) was stained with 5μL monoclonal CD41-PE antibody and 5μL Annexin-V-FITC and Annexin-V binding buffer (1x concentrate) was added to examine the PS-expression of the plaets.

文献2，Blank T et al. The phosphoprotein DARPP-32 mediates cAMP-dependent potentiation of striatal N-methyl-D-aspartate responses. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14859-64. PMID: 9405704

体外研究：

细胞类型（Cell type）：Xenopus Oocytes

药物配制（Preparation）：Calyculin A was prepared in ethanol as a 1 mM stock solution. Immediay after dilution in distilled water to the appropriate concentration, calyculin A was microinjected into the oocytes according to the RNA injection protocol.

实验方法（Assay）：The NMDA-induced currents of striatal mRNA-injected oocytes were measured before and 8 min after 1-min application of 50 μM forskolin or before and 12 min after 1-min application of 10 nM PMA. The same protocol was followed after injecting oocytes with calyculin A to a final intracellular concentration of 500 nM 60–90 min before voltage-clamping. The intracellular concentration of calyculin A was calculated by assuming standard oocytes with a volume of 0.5 μl.

货号

名称

规格

价格（元）

MZ2502-25UG

Calyculin A 花萼海绵体诱癌素A

25 µg

1446

MZ2401-100UL

PMA (TPA, 5mg/ml) 佛波肉荳蔻醋酸（5mg/ml）

100μl

280

MZ2403-1MG

4α-PMA (4α-TPA)  4α-佛波肉荳蔻醋酸

1mg

998