脱氧胞苷激酶抗体用途

中文名称    脱氧胞苷激酶抗体    
英文名称    DCK
别    名    DCK; dCK; DCK protein; DCK_HUMAN; Deoxycytidine kinase; EC 2.7.1; EC 2.7.1.74; MGC117410; MGC138632; OTTHUMP00000219118; OTTHUMP00000219119.    
规    格    0.2ml      
研究领域    肿瘤  染色质和核信号  信号转导  细胞周期蛋白  激酶和磷酸酶      
抗体来源    Rabbit    
克隆类型    Polyclonal    
交叉反应    Human, Mouse, Rat, Pig, Horse,     
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 脱氧胞苷激酶抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    31kDa    
性    状    Lyophilized or Liquid    
浓    度    1mg/1ml    
免 疫 原    KLH conjugated synthetic peptide derived from human DCK    
亚    型    IgG    
纯化方法    affinity purified by Protein A    
储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

脱氧胞苷激酶抗体产品介绍    background:
Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.

Function:
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

Subunit:
Homodimer.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.

Similarity:
Belongs to the DCK/DGK family.

Database links:
UniProtKB/Swiss-Prot: P27707.1

important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.