Features
The new generation of anion exchanger – ultra-fast, transfection-grade plasmid DNA with high yield
• Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
• Column filter included – high filter flow rates, parallel lysate clearing, and loading onto the column
• Improved silica material – typical yields of 400 μg (Midi) and 1 000 μg (Maxi)
• Proven anion-exchange technology – transfection-grade plasmid DNA
• NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation
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NucleoBond® Xtra Midi
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NucleoBond® Xtra Maxi
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Technology
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Anion-exchange chromatography
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Anion-exchange chromatography
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Format
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Midi gravity flow columns
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Maxi gravity flow columns
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Lysate clarification
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Column filters
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Column filters
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Sample material
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< 200 mL (high copy)
< 400 mL (low copy)
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< 600 mL (high copy)
< 1200 mL (low copy)
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Vector size
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< 300 kbp
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< 300 kbp
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Typical yield
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400 μg
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1000 μg
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A260/A280
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1.80–1.95
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1.80–1.95
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Isopropanol precipitation
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Xtra Midi
Centrifugation
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Xtra Midi Plus
NucleoBond®Finalizer
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Xtra Maxi
Centrifugation
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Xtra Maxi Plus
NucleoBond®Finalizer Large
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Preparation time
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70 min/prep
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30 min/prep
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75 min/prep
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35 min/prep
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Ordering information
Product
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Preps
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Specification
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REF
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NucleoBond庐聽Xtra Midi kits
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|
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NucleoBond庐聽Xtra Midi
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10
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NucleoBond庐聽Xtra Midi Columns with inserted NucleoBond庐聽Xtra Column Filters, buffers, RNase A
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740410.10
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50
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as above
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740410.50
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100
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as above
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740410.100
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NucleoBond庐聽Xtra Midi聽Plus
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10
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NucleoBond庐聽Xtra Midi Columns with inserted NucleoBond庐聽Xtra Column Filters,聽NucleoBond庐聽Finalizers, buffers, RNase A
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740412.10
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50
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as above
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740412.50
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NucleoBond庐聽Xtra Maxi kits
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|
|
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NucleoBond庐聽Xtra Maxi
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10
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NucleoBond庐聽Xtra Maxi Columns with inserted NucleoBond庐聽Xtra Column Filters, buffers, RNase A
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740414.10
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50
|
as above
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740414.50
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100
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as above
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740414.100
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NucleoBond庐聽Xtra Maxi聽Plus
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10
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NucleoBond庐聽Xtra Maxi Columns with inserted NucleoBond庐聽Xtra Column Filters,聽NucleoBond庐聽Finalizers Large, buffers, RNase A
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740416.10
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50
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as above
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740416.50
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|
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|
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Product accessories
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Pack of
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Specification
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REF
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NucleoBond庐聽Xtra Combi Rack
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1
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for use with NucleoBond庐聽Xtra Midi, Maxi, BAC,聽AX 100, 500,聽2000, and 10000, BAC 100聽Columns
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740415
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NucleoBond庐聽Xtra Buffer Set I
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1
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for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,聽
only applicable with NucleoBond庐聽Xtra kits, sufficient for 10 Xtra Maxi preps or 20 Xtra Midi preps. Set includes 150 mL聽buffers RES, LYS, NEU, and RNase A
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740417
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