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更新时间:2018-06-12
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Catalogue Number | PRO-182 |
Synonyms | Regulator of G-protein Signaling 2 24kDa, G0S8, G0/G1 switch regulatory protein 8, Cell growth-inhibiting gene 31 protein, RGS2. |
Introduction | RGS2 belongs to the RGS family which are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. RGS2 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits and as a result driving them into their inactive GDP-bound form. |
Description | RGS2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 26.5 kDa. RGS2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | The RGS2 is supplied as a sterile filtered clear solution. |
Formulation | The RGS2 protein solution (0.25mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 2mM DTT and 10% glycerol. |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MQSAMFLAVQ HDCRPMDKSA GSGHKSEEKR EKMKRTLLKD WKTRLSYFLQ NSSTPGKPKT GKKSKQQAFI KPSPEEAQLW SEAFDELLAS KYGLAAFRAF LKSEFCEENI EFWLACEDFK KTKSPQKLSS KARKIYTDFI EKEAPKEINI DFQTKTLIAQ NIQEATSGCF TTAQKRVYSL MENNSYPRFL ESEFYQDLCK KPQITTEPHA T |
Purity | RGS2 purity was found to be greater than 90% as determined by SDS-PAGE. |
Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
Usage | ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |