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价格:电议
所在地:湖北 武汉市
型号:26109
更新时间:2023-04-16
浏览次数:675
公司地址:武汉东湖新技术开发区高新大道666号武汉国家生物产业基地项目(生物创新园)B.C.D区研发楼B3-3楼301室
付兰兰(先生)
货号: 26109 |
基因符号: BRAF, BRAF1, RAFB1 |
描述: Anti-BRaf(N581S) Mouse Mo |
背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
免疫原: A synthetic peptide from the internal region of Braf (N581S), human origin |
经过测试的应用: ELISA, WB, IHC, IP |
推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 2000 IHC: 1: 100-200 |
浓度: 0.5 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Mo |
亚型: IgG |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes B-Raf N581S, but not wild-type B-Raf proteins from vertebrates |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant B-raf proteins.
Purified His-tagged BRaf(N581S) protein (amino acids 513-693, lane 2) and correspo
免疫荧光:
Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(N581S) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmi |