TXB2试剂盒说明书

Mouse Thromboxane B2 (TXB2) ELISA Kit.The kit assay Mouse TXB2 level in the sample，use Purified Mouse TXB2 antibody to coat microtiter plate wells, make solid-phase antibody, then add TXB2 to wells, Combined TXB2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.